Abstract:ObjectiveTo explore the changes of depressive-like behavior, pain threshold and the expression of 5-HT and 5-HT2A receptor mRNA and BDNF mRNA in hippocampus of rats with inflammatory pain and the intervention of duloxetine,and study the relationship between nerve material change in hippocampus and inflammatory pain.MethodsFifty-four adult male SD rats were divided into A,B and C group by random number table,18 rats in each group.Processing of each groups:group A (plantar injection of saline solution),B group (inflammatory pain model),C group(inflammatory pain model+ duloxetine by intragastric administration).The inflammatory pain models were established by subcutaneously injecting mixture of CFA and saline into the left plantar of the rats.Duloxetine [10mg/(kg·d)] was delivered to rats by intragastric administration for 2 weeks.The alterations of 50% paw withdrawal threshold (PWT) were measured before and 2 weeks after CFA injection with Von Frey Fibres.The depression-like behaviors tested with sucrose preference test.While the levels of 5-HT2A receptor mRNA and BDNF mRNA in the hippocampus were detected by RT-PCR and the expression of 5-HT in the hippocampus was detected by ELISA.ResultsBefore treatment,the 50% PWT of the three groups were [(52.60±1.60)g],[(52.93±1.95)g],[(51.47±2.59)g],the difference had no statistical significance (F=0.81,P=0.46).Two weeks later,50%PWT [(41.97±3.88)g] and sugar consumption rate [(80.12±2.91)%] of the group B lower than those of the group A [(49.30±3.20)g,(87.33±5.22)%],the differences had statistical significance (LSDt=2.46,3.21;P=0.03,0.01).Compared with the group B,the 50%PWT [(49.83±7.39)g] and sugar consumption rate [(85.52±3.13)%] in the group C were higher [LSD-t=2.64,2.40;P=0.02,0.03].Compared with A group,5HT of B group [(1.69±0.26)ng/mg;(2.27±0.30)ng/mg] in hippocampus was decreased (LSD-t=2.38,P=0.03),the expressions of 5HT2A receptor mRNA of the two groups (0.62±0.28;1.20±0.30) in the hippocampus had no statistical significance (LSDt=1.57,P=0.14),the expression of BDNF mRNA of group B (0.68±0.29;1.58±0.60) was lower (LSD-t=2.46,P=0.03).Compared with group B,the 5-HT of group C [(2.82±0.62)ng/mg] in hippocampus was increased (LSD-t=4.61,P=0.00),the expressions of 5-HT2A receptor mRNA (1.70±1.04) and BDNF mRNA (1.75±0.88) of group C in hippocampus were higher (LSD-t=2.91,2.91;P=0.01,0.01).ConclusionsThe pain threshold decrease and depression-like behavior appears,and the expression of 5-HT,5-HT2A receptor mRNA and BDNF mRNA in hippocampus decrease in the rat with chronic inflammatory pain.Duloxetine can improve pain threshold and depression-like behavior of the rat with chronic inflammatory pain by increasing the expression of 5-HT,5-HT2A receptor mRNA and the BDNF mRNA in hippocampus.
张焕丽 王微 . 度洛西汀对炎性疼痛大鼠海马5-羟色胺5-羟色胺2A受体基因和脑源性神经营养因子基因表达的干预作用[J]. 中华诊断学电子杂志, 2017, 5(2): 135-.
Zhang Huanli,Wang Wei. Intervention of duloxetine in the expression of 5-HT and 5-HT2A receptor mRNA and BDNF mRNA in hippocampus of rats with inflammatory painZ. zhzdx, 2017, 5(2): 135-.