Abstract：ObjectiveTo study the effects of vibrations on levels of lipid peroxidation and DNA in spermatozoa and the diagnostic significance of vibrations disease.MethodsMale adult rabbits were randomly assigned to control group and three vibration groups,after exposed to vibration for 30 days,semen ROS and SOD were detected by chemiluminescence method and xanthine oxidase method,TUNEL and comet assay test were used to detect the damage of sperm DNA.ResultsCompared with control group,after exposed to vibration for 30 days,the levels of semen ROS were much higher［(A group:（34.43±2.56）RLU/s,B group:（51.37±3.86）RLU/s,C group:（59.74±5.64）RLU/s，F=134.42，P<0.05］,levels of semen SOD were much lower［A group:（51.03±3.81）nU/ml,B group:（34.60±2.39）nU/ml,C group:（32.93±3.87）nU/ml,F=139.60,P<0.05］,the percentage of spermatozoa with damaged DNA were significant higher ［TUNEL：A group:（7.25±1.42）%,B group：（10.33±1.37）%,C group:（4.75±2.30）%,F=56.86,P<0.05;comet assay test：A group:（7.08±1.39）%,B group:（10.58±1.08）%,C group:（15.17±1.90）%,F=90.57,P<0.05］.ConclusionThe vibration may through improve ROS levels and decrease SOD levels to damage spermatozoa DNA,thereby affecting the male reproduction.
祝海洲 沈大庆 张兆强 孟琳. 后肢振动对雄性新西兰兔精浆脂质过氧化指标及精子DNA的影响[J]. 中华诊断学电子杂志, 2015, 3(1): 41-44.
Zhu Haizhou,Shen Daqing,Zhang Zaoqiang,Meng Lin. Effects of hind legs vibration on lipid peroxidation and DNA in spermatozoa of New Zealand rabbits. zhzdx, 2015, 3(1): 41-44.
