Expression of CXCL12/CXCR4,Topo Ⅱ α and NF-κB in tissues of triple negative breast carcinoma
Hu Dongyu1,Xu Fangfang2,Wang Quanyi3,Zhao Guangzhang4,Jiang Xinchang1
1Department of Oncology,3Department of Pathology,4Department of Breast Thyroid Surgery,Affiliated Hospital of Jining Medical University，Jining 272029，China;2Department of Pathology,The First People′s Hospital of Jining,Jining 272011,China
Abstract：ObjectiveTo explore the meaning of the expression of CXCL12/CXCR4,Topo Ⅱα,NF-κB in tissues of triple negative breast carcinoma (TNBC).MethodsFifty-three cases with TNBC were enrolled as TNBC group,and seventy-six cases with non-triple negative breast carcinoma (N-TNBC) as N-TNBC group.Immunohistochemical stainings were conducted with resection specimens,and proteins of CXCL12/CXCR4,Topo Ⅱ α,NF-κB were detected.Patients were followed up after surgery to record postoperative recurrence,metastasis and death.The death cases were recorded by censored data,and surviving cases were followed up for 3 years at least.Rates of recurrence and metastasis,mortality,and disease free survival time (DFS) were compared among different groups.ResultsLess than or equal to 50 years old,lymph node metastasis,invasive ductal carcinoma and Ⅲ stage were TNBC patients′ main features.Positive cells of CXCL12,CXCR4,Topo Ⅱ α,NF-κB were 66.04%,84.91%,69.81%,79.25% respectively in TNBC group,and were 40.79%,51.32%,40.79%,51.32% in NTNBC group.Positive cells of CXCL12,CXCR4,Topo Ⅱ α,NF-κB in TNBC group were all higher than those in N-TNBC group (χ2=7.97,15.51,10.55,10.43;P＜0.05).Metastasis rate,mortality and DFS were 20.75%，28.30%，（24.35±3.50）months respectively in TNBC group,and were 11.32%,13.16%,（32.02±4.00）months in N-TNBC group.Metastasis rate,mortality in TNBC group were both greater than those in N-TNBC group (χ2=4.51,4.58;P＜0.05),yet DFS was less than that in N-TNBC group (t=5.29,P＜0.05).Rates of recurrence and metastasis in positive expression groups of CXCL12,CXCR4,Topo Ⅱ α,NF-κB were 19.70%,17.86%,19.12%,18.52%,and were 6.35%,4.44%,6.56%,4.17% in negative expression groups,and they were all more significant than those in negative expression groups (χ2=5.02,4.61,4.43,5.43;P＜0.05).Mortality was 27.27%,25.00%,26.47%,24.69% in positive expression groups, and was 11.11%,8.89%,11.48%,10.42% in negative expression groups, and they were all bigger than those in negative expression groups (χ2=5.39,4.87,4.63,3.93;P＜0.05).DFS were （23.17±3.30）months, （24.50±3.21）months,（23.51±3.65）months,（25.36±3.65）months in positive expression groups, and were（32.25±3.55）months,（33.00±4.15）months,（35.00±5.10）months,（31.86±4.57）months in negative groups,and the differences between the two groups were statistically significant (t=5.37,5.80,6.21,5.08;P＜0.05).ConclusionThere are high expression of CXCL12,CXCR4,Topo Ⅱ α,NF-κB in tissue of TNBC,with early recurrence and metastasis and poor prognosis,and it may be related with increasing tumor cells′ invasive ability and mediating specific metastasis of lymph node.
