Abstract：ObjectiveTo explore the modulation and mechanism of Apelin-13 in an acute pain model.MethodsThe pain thresholds were continuously monitored in 60min after intracerebroventricular (ICV) administration of Apelin-13 (0.05,0.1 and 0.5μg/g) and casein kinase 2(CK2) inhibitor 4,5,6,7-tetrabromoben:zotriazole (TBB) by radiant heat tail-flick test.Then the expression of CK2 and phosphorylation of NR2B S1480 in hippocampus were detected by Western Blot.ResultsICV injection of Apelin13 induced a dose and time-dependent antinociceptive effect.This effect was signi-cantly antagonized by CK2 inhibitor TBB.Western Blot showed that Apelin-13 could significantly promote the expression of CK2 and the phosphorylation of NR2B S1480,which decreased obviously by TBB.ConclusionApelin-13 plays an antinociceptive role in the acute pain model of radiant heat tail-flick test via modulating CK2 expression and the phosphorylation of NR2B S1480.
