Abstract：ObjectiveTo explore the changes of depressive-like behavior, pain threshold and the expression of 5-HT and 5-HT2A receptor mRNA and BDNF mRNA in hippocampus of rats with inflammatory pain and the intervention of duloxetine,and study the relationship between nerve material change in hippocampus and inflammatory pain.MethodsFifty-four adult male SD rats were divided into A,B and C group by random number table,18 rats in each group.Processing of each groups:group A (plantar injection of saline solution)，B group (inflammatory pain model),C group(inflammatory pain model+ duloxetine by intragastric administration).The inflammatory pain models were established by subcutaneously injecting mixture of CFA and saline into the left plantar of the rats.Duloxetine ［10mg/(kg·d)］ was delivered to rats by intragastric administration for 2 weeks.The alterations of 50% paw withdrawal threshold (PWT) were measured before and 2 weeks after CFA injection with Von Frey Fibres.The depression-like behaviors tested with sucrose preference test.While the levels of 5-HT2A receptor mRNA and BDNF mRNA in the hippocampus were detected by RT-PCR and the expression of 5-HT in the hippocampus was detected by ELISA.ResultsBefore treatment,the 50% PWT of the three groups were ［（52.60±1.60）g］,［（52.93±1.95）g］,［（51.47±2.59）g］,the difference had no statistical significance (F=0.81,P=0.46).Two weeks later,50%PWT ［（41.97±3.88）g］ and sugar consumption rate ［（80.12±2.91）%］ of the group B lower than those of the group A ［（49.30±3.20）g,（87.33±5.22）%］，the differences had statistical significance （LSDt=2.46，3.21；P=0.03，0.01）.Compared with the group B，the 50%PWT ［（49.83±7.39）g］ and sugar consumption rate ［（85.52±3.13）%］ in the group C were higher ［LSD-t=2.64，2.40；P=0.02，0.03］.Compared with A group,5HT of B group ［（1.69±0.26）ng/mg；（2.27±0.30）ng/mg］ in hippocampus was decreased （LSD-t=2.38，P=0.03），the expressions of 5HT2A receptor mRNA of the two groups （0.62±0.28；1.20±0.30） in the hippocampus had no statistical significance （LSDt=1.57，P=0.14）,the expression of BDNF mRNA of group B （0.68±0.29；1.58±0.60） was lower （LSD-t=2.46，P=0.03）.Compared with group B，the 5-HT of group C ［（2.82±0.62）ng/mg］ in hippocampus was increased （LSD-t=4.61，P=0.00），the expressions of 5-HT2A receptor mRNA （1.70±1.04） and BDNF mRNA （1.75±0.88） of group C in hippocampus were higher （LSD-t=2.91，2.91；P=0.01，0.01）.ConclusionsThe pain threshold decrease and depression-like behavior appears,and the expression of 5-HT,5-HT2A receptor mRNA and BDNF mRNA in hippocampus decrease in the rat with chronic inflammatory pain.Duloxetine can improve pain threshold and depression-like behavior of the rat with chronic inflammatory pain by increasing the expression of 5-HT,5-HT2A receptor mRNA and the BDNF mRNA in hippocampus．
张焕丽 王微 . 度洛西汀对炎性疼痛大鼠海马5-羟色胺5-羟色胺2A受体基因和脑源性神经营养因子基因表达的干预作用[J]. 中华诊断学电子杂志, 2017, 5(2): 135-.
Zhang Huanli,Wang Wei. Intervention of duloxetine in the expression of 5-HT and 5-HT2A receptor mRNA and BDNF mRNA in hippocampus of rats with inflammatory painZ. zhzdx, 2017, 5(2): 135-.