Expression and functional changes of KCNQ channels in the chronic compression of dorsal root ganglion model
Li Lei1,Zhang Yue2
1Department of Diagnosis,Jining Medical University,Jining 272067,China；2Department of Board of Health,the first People′s Hospital of Jining,Jining 272100,China
Abstract：ObjectiveTo explore the changes of expression and activity of the KCNQ channels in the chronic compression of dorsal root ganglion (CCD) of SD rats model.MethodsThe von Frey filaments were used to assess pain threshold of CCD rats and the control rats.Real-time polymerase chain reaction (PCR) was performed to determine KCNQ2/3/5 mRNA expression of dorsal root ganglion(DRG) tissue.Western blot assay was used to measure KCNQ2/3/5 protein expression.Whole-cell voltage clamp was used to determine M current density of DRG neurons.ResultsCCD rats showed pronounced hyperalgesia behaviors to noxious mechanical stimulation ［（4.02±1.06）g,（13.31±1.54）g；t=20.46,P＜0.01］.The mRNA and protein levels of KCNQ2/3/5 of DRG tissue notably decreased,followed with the reduction of M current density in DRG neurons of CCD rats ［（5.58±1.34）pA/pF,（7.91±1.52）pA/pF；t=4.62,P＜0.01］.Intragastric administration of retigabine (KCNQ channels opener) to CCD rats alleviated mechanical hyperalgesia ［（6.22±1.52）g,(3.90±1.14)g；t=2.57,P＜0.05］.ConclusionsThe findings elucidated downregulation of the expression and activity of KCNQ2/3/5 channels in DRG neurons of CCD rats contributes to hyperalgesia.Retigabine exhibits an analgesic effect on mechanical allodynia by increasing the M current of DRG neurons of CCD rats.
李雷1张越2. 慢性神经根痛大鼠背根神经节KCNQ通道表达及功能改变[J]. 中华诊断学电子杂志, 2017, 5(3): 208-212.
Li Lei1,Zhang Yue2. Expression and functional changes of KCNQ channels in the chronic compression of dorsal root ganglion model. zhzdx, 2017, 5(3): 208-212.
