Association of mammalian target of rapamycin gene locus rs1883965 polymorphism with kawasaki disease
Chen Fang1, Luo Jie2, Li Shentang2, Luo Yeping2, Yang Zuocheng2
1Department of Pediatrics, Changsha Central Hospital, Changsha 410004, China;2Department of Pediatrics, the 3rd Xiangya Hospital Affiliated to Central South University, Changsha 410013, China
Abstract:ObjectiveTo investigate the genetic association of mammalian target of rapamycin (mTOR) gene locus rs1883965 polymorphism with kawasaki disease (KD). MethodsThis is a case-control study, 100 patients with KD were enrolled from inpatients and follow-up outpatients who visited the Third Xiangya Hospital of Central South University during January 2012 to December 2015, and 100 healthy subjects were enrolled from the health management center of the Third Xiangya Hospital of Central South University in this study. The genotype of locus rs1883965 of mTOR was detected by polymerase chain reaction and gene sequencing. ResultsFor locus rs1883965 polymorphism in mTOR gene, there were no significant differences between KD patients and the controls in genotype frequencies of CC, CT and TT (81.0%, 19.0%, 0%; 85.0%, 14.0%, 1.0%), and allele frequencies of C and T (90.5%,9.5%;92.0%,8.0%) (χ2=1.85,0.28,all P>0.05). For locus rs1883965 polymorphism in mTOR gene, KD patients with coronary artery lesion (CAL) in genotype frequencies of CC, CT and TT (80.0%,20.0%,0.0%) and allele frequencies of C and T(90.0%,10.0%), and KD patients without CAL in genotype frequencies of CC, CT and TT (81.2%,18.8%,0%) and allele frequencies of C and T(90.6%,9.4%). There were no significant differences between the two groups (χ2=0.02,0.02,all P>0.05). ConclusionNo association is found between rs1883965 polymorphism in mTOR gene and the risk of KD and its complication of CAL in this study.
陈芳1罗颉2李申堂2罗叶萍2杨作成2. 哺乳动物雷帕霉素靶蛋白基因rs1883965位点多态性与川崎病的关联性研究[J]. 中华诊断学电子杂志, 2018, 6(3): 172-176.
Chen Fang1, Luo Jie2, Li Shentang2, Luo Yeping2, Yang Zuocheng2. Association of mammalian target of rapamycin gene locus rs1883965 polymorphism with kawasaki disease. zhzdx, 2018, 6(3): 172-176.