Abstract:ObjectiveTo investigate the effects of Apelin-36 on feeding behavior and gastrointestinal motility of rats and its possible pathway. MethodsThere were 88 healthy adult male SD rats in total. Thirty-two rats were randomly selected by random number table and got intracerebroventricular (ICV) injection with saline or Apelin-36(10nmol/L) 3μl, 6μl and 9μl after lateral ventricular catheterization, with 8 rats in each group. The accumulative total of food intake per body weight for 48 hours were measured and calculated. Another 16 rats were randomly selected by random number table, and the gastric emptying rates were calculated in the 16 rats with lateral ventricle catheterization and injection of saline or Apelin-36 (10nmol/L) 9μl, with 8 rats in each group. Sixteen rats were randomly selected by random number table and water sacs were placed in the descending duodenum and baroreceptors were connected. Duodenal motility changes were detected in vivo after lateral ventricular injection of saline or Apelin-36, with 8 rats in each group. Another 16 rats were randomly selected by random number table and colon motility changes were measured in vivo by the same method, with 8 rats in each group. In addition, smooth muscle strips of stomach, duodenum and colon from 8 rats randomly selected by random number table who were dissected to detect the effects of saline or Apelin-36 on the smooth muscle movement in vitro. ResultsThe accumulative food intake/body weight of Apelin-36 3μl, 6μl and 9μl were (147.75±33.06)g/Kg, (127.69±23.94)g/Kg, (99.91±18.48)g/Kg respectively, less than that of saline injected group [(16084±2851)g/kg](F=799,P<001). Compared with saline and Apelin36 3μl group, the food intake of Apelin369μl group was significantly lower (t=6.49, 5.10, all P<0.01). The accumulative food intake/body weight at night of Apelin-369μl group was (45.08±11.86)g/Kg, significantly less than that of saline injected group [(70.77±23.23)g/Kg] (t=4.44, P<0.05). The gastric emptying rate of rats injected with Apelin.36 was (68.10±6.03)%, which was significantly lower than that of saline injected group [(79.21±7.94)%](t=3.15, P<0.01). After ICV injection of Apelin-36, the motive amplitude of descending duodenum decreased to (0.29±0.08)g, significantly lower than that of saline injected group [(0.81±0.16)g] (t=8.36, P<0.01). While there was no significant difference of the motive amplitude of distal colon between Apelin-36 and saline injected group [(t=0.31, P>0.05)]. Apelin-36 showed no significant effects on gastric [(0.19±0.06)g], duodenal [(0.09±0.02)g] and colonic [(0.07±0.01)g] smooth muscle movement in vitro compared with saline [(0.19±0.06)g,(0.08±0.01)g,(0.06±0.02)g] (t=0.13, 0.22, 0.41, all P>0.05). ConclusionIntracerebroventricular injection of Apelin-36 could reduce food intake, inhibit gastric emptying and duodenal motility, while Apelin-36 shows no direct effect on gastrointestinal smooth muscle movement in vitro.
武菲 崔孟颖 陈岳彤 高幸 李璐 冯其贞 白波. Apelin-36通过中枢途径对大鼠摄食、胃肠运动调节作用的研究[J]. 中华诊断学电子杂志, 2020, 8(1): 62-67.
Wu Fei, Cui Mengying, Chen Yuetong, Gao Xing, Li Lu, Feng Qizhen, Bai Bo. Regulatory effect of Apelin-36 on food intake and gastrointestinal motility in rats via central pathway. zhzdx, 2020, 8(1): 62-67.