Abstract:ObjectiveTo investigate the characteristics and detection methods of secondary agglutination of cross matching in children with polyagglutination of red blood cells (RBCs). MethodsThe detection results of 8 child cases with cross matching secondary hemagglutination from January to December 2019 in Blood Group Reference Laboratory of Jining Blood Center were retrospectively analyzed. After ABO and Rh blood type identification, direct antiglobulin test (DAT), antibody screening and cross matching methods, false agglutination was excluded. Polyagglutinated RBCs were confirmed by 3 normal adults of homotypic blood serum, 3 normal adult AB serum and peanut agglutinin. ResultsIn the 8 cases, the DAT of RBCs antibody screenings and the primary side of cross matching blood were negative. While the results from the secondary saline method and anti-human globulin method were positive. The RBCs of the children were agglutinated with AB serum and peanut agglutinin. All the elements showed an agglutination reaction, and the saline was used to eliminate false agglutination like rouleaux. ConclusionsThe polyagglutinated RBCs agglutinate with most adults sera, which lead to secondary positive in cross matching test. Confirming the polyagglutination of erythrocytes will help to identify the causes of cross matching difficulties and avoid delaying transfusion for child patients.
孔凡生 耿微. 多凝集红细胞致患儿交叉配血次侧凝集的诊断特征分析[J]. 中华诊断学电子杂志, 2021, 9(3): 187-191.
Kong Fansheng, Geng Wei. Diagnostic feature analysis of secondary agglutination of cross matching in children caused by polyagglutination of red blood cells. zhzdx, 2021, 9(3): 187-191.