Correlation between CTSB gene promoter region polymorphism and clinical features in patients with acute myocardial infarction
Kong Qian1, Bai Guannan2, Zhou Yu1, Yan Bo3
1College of Clinical Medicine, Jining Medical University, Jining 272013, China; 2Cheeloo College of Medicine, Shandong University, Jinan 250012, China; 3Shandong Provincial Laboratory of Cardiac Disease Diagnosis and Treatment, Affiliated Hospital of Jining Medical University, Jining 272029, China
Abstract:ObjectiveTo investigate the relationship between acute myocardial infarction (AMI) and single nucleotide polymorphism (SNP) of cathepsin B(CTSB) gene promoter region rs1293312T/C site and the associated risk factors. MethodsA casecontrol study was performed to collect 201 AMI patients (AMI group) hospitalized in the Department of Cardiology, Affiliated Hospital of Jining Medical University from January 2021 to December 2021, and 204 healthy controls (control group) in the physical examination center. Following DNA sequencing data were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCRRELP) technique combined with sequence alignment. Following the Hardy-Weinberg balance test, the χ2 test was used for correlation analysis. Logistic regression was utilized to examine the relationship between various risk variables, SNP loci and the incidence of AMI. ResultsThe SNP loci revealed three genotypes, including CC, CT and TT. HardyWeinberg equilibrium (χ2=0.57, 1.68, all P>0.05) and the genotype distribution were both supported by the data. There were no significant differences (χ2=0.15, 0.01, all P>0.05) between the CC, TC, TT genotypes of the AMI group [34(16.92%), 91(45.27%), 76(37.81%)] and the control group [37(18.14%), 89(43.63%), 78(38.23%)], as well as the C and T alleles of the AMI group [159(39.55%), 243(60.45%)] and control group [163(39.95%), 245(60.05%)]. Logistic regression analysis in five different genetic models suggested that the SNP loci was not associated with the onset of AMI (all P>0.05). ConclusionThe population genetics of the CTSB gene promoter region polymorphism is provided by the CTSB gene promoter rs1293312 T/C SNP, which is not related to the onset of AMI.
孔倩 白冠男 周瑜 闫波. 组织蛋白酶B基因启动子多态性与急性心肌梗死发病的关系[J]. 中华诊断学电子杂志, 2022, 10(3): 171-176.
Kong Qian1, Bai Guannan2, Zhou Yu1, Yan Bo3. Correlation between CTSB gene promoter region polymorphism and clinical features in patients with acute myocardial infarction. zhzdx, 2022, 10(3): 171-176.