5-氟尿嘧啶磁性白蛋白微球对离体牛肝脏射频消融增效作用的研究

doi:10.3877/cma.j.issn.2095-655X.2014.03.009

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摘要目的探讨射频消融（RFA）联合5氟尿嘧啶磁性白蛋白微球（5FuMAMS）对离体牛肝脏射频消融体积的影响。方法实验材料采用新鲜离体牛肝，根据消融介质的不同，随机数字表法分为3组：A组为单纯RFA组；B组为RFA联合游离5Fu组；C组为RFA联合5FuMAMS组。观察各组消融灶形态学特征，测量消融灶平均最大纵径和横径，计算消融灶体积，通过肉眼大体观察和显微镜下观察消融灶病理改变。射频消融时间、温度、消融灶纵径、消融灶横径以均数±标准差表示，显著性检验采用单因素方差分析。结果离体牛肝对射频融灶的形态肉眼观，所有消融灶中心横切面大体形态均呈灰白色椭圆形外观；剖面可见明显的凝固性坏死区，中央组织干燥，治疗区域和周围肝组织分界比较清楚；镜下呈典型凝固性坏死改变。A、B、C组所形成的平均消融体积最大纵径分别为（4.45±0.23）cm、（4.93±0.53）cm、（5.68±0.59）cm，差异有统计学意义（F＝10.23，P<0.05）；平均最大横径分别为（2.83±0.25）cm、（3.08±0.22）cm、（4.10±0.14）cm，差异有统计学意义（F＝61.23，P<0.05）， RFA联合5FuMAMS组平均消融体积明显大于其他两组。A、B、C组平均消融时间分别为：（472.00±20.49)s、(632.50±21.05)s、(836.50±19.10)s，差异有统计学意义(F＝489.28，P<0.05)。A、B、C组消融完成时距离中心穿刺点周围3cm处的平均温度变化分别为：(41.83±2.04)℃、(47.33±3.83)℃、(55.67±2.16)℃，差异有统计学意义(F＝37.20，P<0.05)。结论RFA联合5FuMAMS，能产生更大的组织消融灶，组织病理上呈典型凝固性坏死改变。

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关键词 ：导管消融术, 氟尿嘧啶, 白蛋白类, 微球体, 动物实验

Abstract：ObjectiveTo investigate the influence of radiofrequency ablation(RFA) combined with 5FuMAMS injection on the ablation volume.MethodsThe subjects used in this experiment were fresh ex vivo bovine livers.Three groups were set up:Group Ⅰ,RFA alone;group Ⅱ,RFA combined with 5fluorouracil;group Ⅲ, RFA combined with 5fluorouracil magnetic albumin microspheres.The size and shape of coagulation necrosis zones were observed and measured.Single factor analysis of variance was used to analyze the affecting factors for the size of ablation lesions.Macroscopic and microscopic morphological characteristics of lesions were observed．ResultsIn gross examination，all ablation lesions were gray elliptical in cross section and there were classical coagulation necrosis.The central organization was drying,and the treatment area and surrounding liver tissue boundaries were clear.The mean biggest longitudinal diameter and transverse diameter of necrosis volume of group I,group II and group III were (4.45±0.23)cm,(4.93±0.53)cm,(5.68±0.59)cm（F＝10.23，P<0.05）and (2.83±0.25)cm, (3.08±0.22)cm, (4.10±0.14)cm respectively（F＝61.23，P<0.05）. The necrosis volumes of group Ⅲ(RFA combined with 5FuMAMS) were bigger than those of control group(P<0.05）．The mean durations were （472.00±20.49)s,(632.50±21.05)s and (836.50±19.10)s (F＝489.28，P<0.05).The average temperature at 3cm distance from the center of the puncture point were (41.83±2.04)℃,(47.33±3.83)℃ and (55.67±2.16)℃ (F＝37.20，P<0.05).All the differences were statistically significant.ConclusionsCompared with other two groups,RFA combined with 5fluorouracil can produce larger ablation zones．The lesions typically showed coagulation necrosis at microscopicaI examination．

Key words： Catheter ablation Fluorouracil Albumins Microspheres Animals experimentation

收稿日期: 2014-08-12

基金资助:山东省自然科学基金资助项目（ZR2010HL052）

通讯作者: 刘建刚，Email：liu517206@163.com

引用本文:

刘建刚乔森袁常秀刘志超崔文陈桂林. 5-氟尿嘧啶磁性白蛋白微球对离体牛肝脏射频消融增效作用的研究[J]. 中华诊断学电子杂志, 2014, 2(3): 202-205.
Liu Jiangang*,Qiao Seng,Yuan Changxiu,Liu Zhichao，Cui Wen,Chen Guilin. The experimental study of 5fluorouracil magnetic albumin microspheres enhancing radiofrequency ablation in ex vivo bovine liver. zhzdx, 2014, 2(3): 202-205.

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http://www.zhzdxzz.com/CN/10.3877/cma.j.issn.2095-655X.2014.03.009 或 http://www.zhzdxzz.com/CN/Y2014/V2/I3/202

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