1Department of General Surgery,Affiliated Rizhao People′s Hospital of Jining Medical College,Rizhao 276826,China；2Department of Internal Medicine,Jufeng Hospital of Rizhao,Rizhao 276812,China;3Department of General Surgery;Rizhao Hospital of TCM,Rizhao 276800,China;4Department of Surgery Teaching and Research Section,Weifang Medical University,Weifang 261053,China
Abstract：ObjectiveTo investigate the expression of cyclooxygenase 2 (COX-2) and transcription factor Ki-67 in colorectal carcinoma and their relationship with invasion and metastasis.MethodsThe expressions of COX-2 and Ki-67 were measured by immunohistochemical method staining in one hundred and twenty cases with colorectal carcinoma,forty cases with dysplasia tissue closely adjacent to carcinomas,and twenty cases with normal colorectal mucosa specimens.All the clinical and follow-up information were reviewed and evaluated.Fisher′s exat test,spearman′s correlation analysis,chi-square test were used to analyze the expressions of COX-2 and Ki-67.ResultsThe expressions of COX-2 and Ki-67 in colorectal cancer tissues（78.3％,73.3%,respectively） were obviously higher than those in adjacent tissues (25.0％,35.0%,respectively) and normal mucosal tissues (10.0%,20.0%,respectively) （COX-2:χ2=18.755，P<0.05；χ2=29.511，P<0.05.Ki67:χ2=9.538,P<0.05;χ2=24.621,P<0.05).The differences were statistically significant .The expressions of COX-2 and Ki-67 in the high grade carcinoma were higher than those in low grade cancer（χ2=6.591,P<0.05;χ2=6.160,P<0.05）,and the expression in advanced cancer was significantly raised compared with early cancer（χ2=4.925，P<0.05；χ2=6.317，P<0.05）.The expression levels of COX-2 were correlated with lymph node metastasis（χ2=6.104，P<0.05;χ2=6.104，P<0.05）.No significant differences were found in the expressions of Ki-67 among different types of high grade cancer had no significant difference（χ2=0.958，P＞0.05）,and there was no significant difference between colorectal dysplasia tissue and normal colorectal mucosa tissue.ConclusionsThe abnormal expressions of COX-2 and Ki-67 plays an important role in the genesis and development of colorectal carcinoma and may be responsible for the enhanced activity in tumorinduced neovascularization,invasion and metastasis.COX-2 and Ki-67 have positively expressive correlation and may serve as valuable indicators of biological behavior of colorectal carcinoma.COX-2 and Ki-67 may be the prognostic discriminators of colorectal carcinoma.
