1Department of Clinical Laboratory,Affiliated Hospital of Jining Medical University,Jining 272029,China;2Department of Biotechnology,Jining Medical University,Jining 272067,China
Abstract:ObjectiveTo explore the influence of room temperature and humidity on the measurement results of hepatitis C virus antibody using high-throughput ELISA method.MethodsTo detect the S/CO values of HCV antibody standard using addcare automatic enzyme immunoassay instrument (ELISA 1100) under 24℃ and different humidity (30%RH,40%RH,50%RH,60%RH,70%RH,80%RH,90%RH) and 50%RH and different temperatures (16℃,18℃,20℃,22℃,24℃,26℃,28℃,30℃,32℃,34℃,36℃,38℃,40℃) respectively.ResultsThe S/CO values of HCV-Ab standard showed significant difference under different temperature (16℃,18℃,20℃,22℃,24℃,26℃,28℃,30℃,32℃,34℃,36℃),and the S/CO value of standard was positively correlated with temperature.The S/CO value altered little between 36℃ and 38℃,but there was of significant difference between 38℃and 40℃.While the S/CO value of the standard gradually decreased with the rising of humidity(30%RH,40%RH,50%RH,60%RH,70%RH,80%RH,90%RH),which presented significant difference between different humidity (P<0.05),and S/CO value of the standard had a negative correlation with the humidity.ConclusionsRoom temperature and humidity have important effects on high-throughput ELISA detection results.High-throughput ELISA should been carried out in the environment with no more than 36℃ and low humidity.Comprehensive consideration is recommended for the automatic enzyme immunoassay instrument with operating temperature range from 18 to 25 degrees,humidity control between 35%RH~50%RH.
董海新1金呈强1赵元明1张健1孙晶1余维星2. 温湿度对高通量酶联免疫吸附实验测定丙型肝炎病毒抗体结果的影响[J]. 中华诊断学电子杂志, 2016, 4(1): 48-.
Dong Haixin1,Jin Chengqiang1,Zhao Yuanming1,Zhang Jian1,Sun Jing1,Yu Weixing2. The influence of room temperature and humidity on the measurement results of hepatitis C virus antibody using highthroughput ELISA method. zhzdx, 2016, 4(1): 48-.