Abstract：ObjectiveTo investigate the effect of 7,8-dihydroxyflavone (7,8-DHF) on the phenotypic transformation of vascular smooth muscle cells (VSMCs) in spontaneously hypertensive rats (SHR). MethodsWestern blotting assay was carried out to detect the Wistar-Kyoto rats and SHR′s protein expression of smooth muscle actin (SM-α-actin) and proliferating cell nuclear antigen (PCNA). With the different concentrations of 7,8-DHF and (or) TrkB specific inhibitor ANA-12, the VSMCs derived from thoracic aortic of SHR were processed and the VSMCs activity was detected by CCK-8 method. Western blotting was used to detect the protein expression levels of SM-α-actin and PCNA after 7,8-DHF and (or) ANA-12 treated in VSMCs derived from thoracic aortic of SHR. The independent sample t-test and single factor variance analysis were used for statistical comparison.ResultsCompared with WKY,the expression of SM-α-actin in aorta of SHR decreased significantly ［(0.72±0.06),(0.41±0.08),t＝6.35，P＜0.05］, while PCNA increased［(0.51±0.09), (0.99±0.15),t＝6.43，P＜0.05］, and so was the case in VSMCs derived from thoracic aortic of SHR, SM-α-actin decreased ［(0.71±0.05), (0.36±0.04),t＝11.12，P＜0.01］ and PCNA increased［(0.69±0.07), (1.05±0.08), t＝8.49，P＜0.05］. The proliferative activity of VSMCs derived from thoracic aortic of SHR was greatly attenuated by 7,8-DHF in a concentration-dependent manner(F＝17.49,P＜0.01), which could be enhanced by ANA-12 ［ (0.52±0.08),(0.86±0.14),t＝5.13,P＜0.01］. ANA-12 can significantly weaken the effect of 7,8DHF on the expression of SM-α-actin［(0.85±0.13),(0.35±0.15), t＝4.37,P＜0.01］and PCNA protein［(0.42±0.13),(0.76±0.10),t＝3.54,P＜0.05］in VSMCs derived from thoracic aortic of SHR. Conclusion7,8-DHF can effectively inhibit the transformation of VSMCs derived from thoracic aortic of SHR from contractile to proliferative type,which may be mediated by TrkB receptor.
姚天成 王伟杰 杨莹莹 郑祥珍 刘海青 王炳香. 7,8-二羟基黄酮对自发性高血压大鼠血管平滑肌细胞表型转化的影响[J]. 中华诊断学电子杂志, 2018, 6(3): 202-206.
Yao Tiancheng, Wang Weijie, Yang Yingying, Zheng Xiangzhen, Liu Haiqing, Wang Bingxiang. Effect of 7,8-dihydroxyflavone on the phenotype transformation of vascular smooth muscle cells in spontaneously hypertensive rats. zhzdx, 2018, 6(3): 202-206.
