Osteopontin gene locus 708C/T polymorphism in children with Kawasaki disease
Li Pengzhu1, Zhan Xia2, Xie Gui1, Liu Xin1, Qian Yujie1, Li Zhuoying1, Huang Lihua3, Yang Zuocheng1
1Department of Pediatrics, 3Department of Center for Medical Experiments, the Third Xiangya Hospital of Central South University, Changsha 410013, China；2Department of Pediatrics, the First People′s Hospital of Changde, Changde 415003, China
Abstract：ObjectiveTo investigate the polymorphism of locus 708C/T in Osteopontin (OPN) gene of children with Kawasaki disease (KD) and the correlation between the polymorphism and coronary artery lesion (CAL). Methods A total of 103 cases of patients with KD hospitalized in pediatrics department of the Third Xiangya Hospital of Central South University from October 2006 to March 2015 and 100 cases healthy children who underwent physical examination in the same period were selected as the study subjects. In the case-control study, polymorphism analysis of locus 708C/T in OPN gene of 203 cases were detected using PCR-RFLP method. According to the presence or absence of CAL, the KD group was divided into KD combined with CAL (n=43) and KD without CAL (n=60). ResultsIn KD group, the 3 kinds of genotype distribution frequencies of OPN gene 708C/T site were CC (8.74%), CT (29.13%), TT (62.14%), and the allelic frequencies were C (23.30%) and T (76.70%), respectively. In normal control group, the 3 kinds of genotype distribution frequencies of OPN gene 708C/T site were CC (8.00%), CT (35.00%), TT (57.00%), and the allelic frequencies were C (25.50%) and T (74.50%). There were no significant differences between the two groups (χ2=0.79, 0.28, all P＞0.05).The frequencies of three genotypes of OPN gene 708C/T site in KD with CAL group were CC (6.98%), CT (34.88%) and TT (58.14%), and the allelic frequencies were C (24.42%) and T (75.58%), respectively. The frequencies of OPN gene 708C/T site in KD without CAL group were CC (10.00%), CT (23.33%) and TT(66.67%), and the allelic frequencies were C (21.67%) and T (78.33%), respectively. There were no significant differences between the two groups (χ2=1.73, 0.22, all P＞0.05). ConclusionNo correlation is found between the polymorphism of locus 708C/T in OPN gene and the occurrence of KD and its complication CAL.
