Changes of protein phosphotase-2A level in lymphocytes of type 2 diabetes mellitus accompained by Alzheimer′s disease
Sun Yinyi1, Wang Shujin2, Liu Bangjian1, Qu Zhongsen1
1Department of Neurology, Shanghai Sixth People′s Hospital East Affiliated to Shanghai University of Medicine & Health Science, Shanghai 201306, China； 2Department of Neurology, the First People′s Hospital of Zibo Affilliated to Weifang Medical College, Zibo 255200, China
Abstract：ObjectiveTo explore the alterations of protein phosphotase-2A (PP-2A) level in lymphocytes in the patients with type 2 diabetes mellitus (type 2 DM) accompained by Alzheimer′s disease(AD). MethodsFrom July 2016 to October 2017， 57 patients in Neurology department of east section, Shanghai Sixth People′s Hospital East and the First People′s Hospital of Zibo were enrolled. All patients were divided into AD group (n=20), type 2 DM (n=20) and type 2 DM accompained by AD group (n=17). Healthy subjects (n=23) of the east section of Shanghai Sixth People′s Hospital for the same period were selected as the control group. The activity of PP2A was determined by 32P-labelling, and the PP-2A expression of each group was measured by western-blotting. ResultsCompared with control group（0.99±0.06，1.02±0.09）， the activity and the expression of PP-2A were decreased in type 2 DM group （0.87±0.11，0.91±0.10）, AD group（0.76±0.13， 0.86±0.11） and type 2 DM accompained by AD group（0.62±0.72，0.71±0.09）, the differences were statistically significant （F=5.27，7.74，all P＜0.05）. Furthermore, the activity and the expression of PP2A in type 2 DM accompained by AD group were lower than those in type 2 DM group （LSDt=7.40，5.85，P＜0.05） and AD group （LSDt=4.56, 4.57，P＜0.05）. The activity of PP-2A in AD group was lower than that in type 2 DM group (LSD-t=2.84, P＜0.05), but the expression of PP-2A between the two groups was not statistically significant (LSD-t=1.28，P＞0.05). ConclusionLower activity and expression of PP-2A level in lymphocytes in type 2 DM accompained by AD patients, implying a possible value for diagnosis and treatment.
孙寅轶 王姝瑾 刘帮健 曲忠森. 2型糖尿病并发阿尔茨海默病患者外周血淋巴细胞蛋白磷酸酯酶-2A的变化[J]. 中华诊断学电子杂志, 2019, 7(2): 78-82.
Sun Yinyi1, Wang Shujin2, Liu Bangjian1, Qu Zhongsen1. Changes of protein phosphotase-2A level in lymphocytes of type 2 diabetes mellitus accompained by Alzheimer′s disease. zhzdx, 2019, 7(2): 78-82.
